Please join us for the monthly meeting on January 24th, 2022 

Rebekah E. Dumm, PhD

CPEP Fellow in Medical and Public Health Microbiology

Hospital of the University of Pennsylvania and

Children’s Hospital of Philadelphia 

Clinical Utility of Broad-Range 16S rRNA gene PCR with Sequencing

Broad-range bacterial 16S rRNA gene PCR with sequencing (BRPS) is used to detect bacterial pathogens present in clinical specimens. BRPS has demonstrated utility in identifying the causative agent, particularly from normally sterile sites with high suspicion of infection, even when cultures are negative. The goal of this study was to review the experience at the Hospital of the University of Pennsylvania (HUP) with send-out testing to reference laboratories for BRPS, evaluating ways to improve clinical utilization of the test. This was accomplished by correlating the BRPS results with microbiology cultures and surgical pathology findings and using these features to predict high-yield samples and specimen types. This talk will also discuss optimal use cases and highlight some practical interventions for improving patient care through diagnostic stewardship.

Lecture 5 to 6pm

Zoom Meeting link will be emailed to Branch members

Live Stream on our YouTube Channel : Click here