Black Spot, Black Death, Black Pearl: Tales of Bacterial Effectors

This lecture uses three interwoven examples to illustrate how bacterial pathogens rewire host cells using their effector proteins. Black Spot refers to work with Xanthamonas campestris, which causes black spots on tobacco and tomato leaves. Black Death highlights work with Yersinia pestis, which causes “the black plaque,” and how Yersinia Yop proteins hijack host cells. Black pearl covers work with effector proteins from Vibrio parahaemolyticus, which contaminates oysters.

More information about the Kim Orth Lab

For those that cannot attend in person, the meeting will be live streamed on Zoom (Members only) or the EPAASM YouTube channel

From the EPAASM archives of previous “Centennial” meetings

118 (Jan 28 1936) – The advantages of vacuum dried complement for use in the routine Wasserman Reaction – Fred Borner, Marguerite Lukens (first record available of a monthly meeting title)

200 (March 23 1948) – The in vivo reactivation by BAL (2,3 dimercapto-propanol) of influenza virus and pneumococci inactivated by bichloride of mercury – Morton Klein, JE Perez

300 (Oct 23 1962) – Genetic alterations of purine pyrophosphorylases – Joseph Gots, GP Kalle, J Adye

The effect of 5-fluoro-2-deoxyuracil on experimental Herpes simplex infection – Claire Engle

The effect of biotin deficiency on the synthesis of nucleic acids, protein and enzymes in yeast – Ahmad Fazal

400 (March 28 1977) – Important pathogenic fungi encountered in the diagnostic laboratory – Fritz Blanc

500 (March 23 1987) – Fungal chemotherapy: novel agents and therapeutic strategies- George Kobayashi

600 (Nov 17 1997) – Helicobacter pylori and the pathogenesis of upper gastrointestinal disorders – Martin Blaser

700 (Sept 28 2009) – Horizontal gene transfer among pathogens during chronic infections serves as a counterpoint to the host’s adaptive immune system – Garth Erlich

800 (Feb 27 2023) – Challenges in the diagnosis of fungal infections and the Fungal Diagnostic Laboratories Consortium – Esther Babady

Application Process and Selection Criteria for the Annual Peggy Award Travel Award

Description: Each year, National ASM awards three travel awards for early career branch members to attend ASM Microbe to the Branch for early career ASM National (contributing or premium) and branch members. Selected recipients will represent EPA-ASM at the microbe meeting. Microbe 2023 will be held Jun 15 – 19, 2023 in Houston, Texas, USA.

Use of Awards: Awardees must use the travel award funding towards costs associated with registration, accommodation, and travel to ASM Microbe.

Definition of Early Career: Nominations must fall into the one of the following Early Career categories for consideration AND be members of the EPA-ASM branch:

· Postdoctoral fellows (up to 12-years beyond terminal degree).

· Clinical Laboratory Scientist -MLT-MLS Bench Techs (up to 12-years beyond terminal degree).

· Early Career Faculty (either tenure track or non-tenure track, e.g., Assistant Professors, Research Assistant Professors, Visiting Assistant Professors, etc.) (up to 12-years beyond terminal degree).

· Early-Stage Investigators (ESI’s) (up to 12-years beyond terminal degree).

· Other – as determined by Branch, but only individuals who are up to 12-years beyond terminal degree.

· NOTE – Students are NOT eligible for this award. Past recipients of this travel award are NOT eligible to receive the award again.

· EXCEPTIONS – Exceptions to the 12-year time limit may be made in special circumstances (career delays, parental leave)

Application Process: EPA ASM Application Form can be completed online at the following link: https://drexel.qualtrics.com/jfe/form/SV_37C1gdbguW7Jyuy

The following information will be required to complete the online application:

· Nominator name (principal investigator or supervisor) for postdocs or Clinical laboratory scientists

· Early Career Faculty name (self-nomination)

· Name of Postdoc or Clinical laboratory scientist

· Credentials/terminal degree

· Year of matriculation of terminal degree

· A link to upload the abstract that was submitted to ASM Microbe Conference

· A short statement (250 words) explaining why the candidate should receive the travel award to attend Microbe and nominee’s contributions to the branch. How will attending the meeting help advance their science and career? This can be written by the nominator/supervisor or by the early career faculty who self-nominated.

· Gender and race/ethnicity of the nominee (with option to not disclose). ASM requests demographic information of awardees.

Selection Process: The EPA-ASM selection committee will be made up of 3 members of the branch executive committee including the (1) President or the President-elect, (2) Council on Microbial Sciences (COMS) member, and a (3) Clinical Microbiology faculty representative. In addition, the selection committee will include 2 officers from the student chapter (unless conflict; for example, the nominated

faculty or postdoc is part of their thesis advisory committee or thesis research mentor). A quorum is required for voting which equals at least 2 faculty members and 1 student member.

Selection Criteria:

· Nominee must be member of ASM National (contributing or premium) and EPAASM members.

· Preference will be given to applicants who are active in the branch (for example: participate in monthly meetings, clinical symposium, Philadelphia Infection, and Immunity Forum (PIIF), or other branch committee work). Preference may be given to faculty members who have given a lecture at the Monthly Meeting Program, postdoctoral fellows who have given an oral presentation at the annual PIIF.

· Considerations may be given to foster diversity, equity, and inclusion.

· While Peggy Cotter Award recipients are not guaranteed poster or oral presentations at ASM Microbe, exceptional abstracts may be given priority.

· Nominees have evidence as a “multiplier/influencer” or display exceptional mentorship.

· While not restricted based on training, to increase the diversity of interests/experience/expertise, EPA-ASM branch aims to award one early career (up to 12-years beyond terminal degree) basic research faculty/clinical microbiologist faculty member, one postdoctoral fellow, and one Medical Laboratory Scientist (MLS)/Clinical Laboratory Scientist (CLS)/Medical Laboratory Technician (MLT).

· Previous Peggy Cotter awardees are not eligible. If you receive another travel award from ASM/Microbe, you would not be eligible to receive the Cotter award.

Application and Selection Timeline:

1. Nominations/Applications accepted: 1/30/23 to 2/20/23 (midnight)

2. Selection Committee reviews applications and meets to select awardees: 2/21/23 through 2/24/23

3. Announcement of Awards: 2/24/23 emails to recipients; awardees must accept award by 2/27/23

4. EPA-ASM awardees are communicated to ASM National by March 1, 2023

Expectation of selected awardees: Awardees are expected to complete a short online survey evaluation of their experience at Microbe after the conference in June, and to give a 5-to-10-minute summary of their science and ASM experience at a future monthly branch meeting. Awardees should acknowledge the EPA-ASM branch on their Microbe poster or oral presentation and consider sharing photos from Microbe and lessons learned through social media (tagging EPA-ASM branch). Awardees ultimately serve as an ambassador of our branch and should be willing to share experiences and lessons learned back to their institution and our branch.