Annual Student Organized Meeting

Keynote Speaker: 5:15 – 6:15 pm

Shantanu Bhatt, Ph.D.
Associate Professor of Biology
Saint Joseph’s University, Philadelphia


“Small regulatory RNAs: big players in the virulence of enteropathogenic E. coli”

Dr. Bhatt studies the bacterial pathogen enteropathogenic Escherichia coli (EPEC). EPEC is a major public health concern in developing countries, where it causes significant morbidity and mortality in infants. The genetic basis for the virulence of EPEC is the locus of enterocyte effacement (LEE). Whereas transcriptional control of the LEE has been well-characterized, post-transcriptional regulation of the LEE has been understudied. So, Dr. Bhatt has focused his career on uncovering how trans-encoded small regulatory RNAs (sRNAs) modulate the expression of the LEE and, thereby, control the virulence of EPEC. He uses a highly interdisciplinary approach by incorporating tools from bioinformatics, genetics, and biochemistry to interrogate and characterize these sRNAs. In addition to bacterial pathogenesis research, Dr. Bhatt is a passionate mentor and teacher. As a professor at Saint Joseph’s University, he teaches students both inside the lab and the classroom.

Program

Student Talks (4:00pm to 5:00pm)

4:00-4:15pm: “Microbial cooperation enhances Clostridioides difficile pathogenesis

Alexander Smith, PhD Candidate in the Zackular lab, University of Pennsylvania Perelman School of Medicine

4:15-4:30pm: “Defining the bacterial and host factors responsible for caspase-1-11-independent cell death during infection by Salmonella enteritidis”

Beatrice Herrmann, PhD Candidate in the Brodsky lab, University of Pennsylvania Perelman School of Medicine

4:30-4:45pm: “Age-associated defects in the adaptive immune response to Clostridium difficile infection impair the development of a protective immune response in the elderly

Matthew Bell, PhD Candidate in the Kutzler lab, Drexel University College of Medicine

4:45-5:00pm: “Microbial-based mechanisms of accelerated wound healing”

Ellen White, PhD Candidate in the Grice lab, University of Pennsylvania Perelman School of Medicine

Keynote Speaker: 5:15 – 6:15 pm

Virtual meeting information to be emailed to branch members
Non-members can livestream the program from the Branch YouTube Channel

Kyle G. Rodino, Ph.D., D(ABMM)
Assistant Professor of Clinical Pathology and Laboratory Medicine
Assistant Director, Clinical Microbiology
Hospital of the University of Pennsylvania and Perelman School of Medicine, University of Pennsylvania

Next-Generation Sequencing in Clinical Microbiology: Current Applications and What’s Next

Advanced molecular diagnostics, including next-generation sequencing (NGS)-based technologies, are increasingly integrating into the clinical microbiology laboratory. These new technologies have the ability to provide information not previously attainable, with current applications including organism identification, outbreak investigation and mutation surveillance, and detection of resistance determinants. Of particular interest to the diagnosis of infectious diseases is the use of metagenomic NGS (mNGS), targeted or unbiased, for pathogen detection direct from patient specimens. Case reports have shown the undeniable power of mNGS to provide diagnostic evidence where other methods have failed. However, a number of hurdles to routine use remain, including technical performance characteristics and clinical utility. Addressing these issues will be important to broad adoption of mNGS methods for infectious disease testing in the clinical microbiology laboratory, particularly as we look toward future applications, including rapid sequencing assays, antimicrobial susceptibility modeling, and disease-predictive host biomarkers.

Fabio Bagnoli, Ph.D.
Project Leader
GSK Vaccines

“Staphylococcus aureus – the immune evader”

Virtual Meeting
5 – 6pm

Zoom meeting details to be emailed to branch members. Non-branch members can watch the live stream on our YouTube Video Channel

upcoming Branch events
May 24 – Student chapter sponsored event, keynote speaker Shantanu Bhatt, Ph.D., St. Joseph’s University
June 14 – Norman Willett Memorial Lecture, keynote speaker Amy Vollmer, Swarthmore College

John Mekalanos, Ph.D.
Adele Lehman Professor of Microbiology & Molecular Genetics
Department of Microbiology
Harvard Medical School

Protein covariance networks reveal interactions important to the emergence of SARS coronaviruses as human pathogens

SARS-CoV-2 is one of three recognized coronaviruses (CoVs) that have caused epidemics or pandemics in the 21st century and that likely emerged from animal reservoirs based on genomic similarities to bat and civet viruses. Here we report the analysis of conserved interactions between amino acid residues in all proteins encoded by SARS-CoV-related viruses. We identified pairs and networks of residue variants that exhibited statistically high frequencies of covariance with each other. While these interactions are likely key to both protein structure and other protein-protein interactions, we have also found that they can be used for understanding viral evolution and adaptation. Our data provide evidence that the evolutionary processes that converted a bat virus into human pathogen occurred through recombination with other viruses in combination with new adaptive mutations important for entry into human cells.

Lecture: 5 – 6pm

Also available on our branch YouTube channel

https://mekalanoslab.med.harvard.edu/